Composite

Part:BBa_K5106006:Design

Designed by: Ivar Gruppen   Group: iGEM24_WageningenUR   (2024-10-01)


Toehold switch C for detection of hsa-miR-484 with T7 promoter and terminator, and LacZ reporter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The T7 promoter was used, as it was compatible with our cell-free system (PURExpress).



Source

Designed by miRADAR

References